Category: Stem Cells

In decided on experiments, cell line supernatants were preincubated for one hour at space temperature with neutralizing antisera to IL-10 (10 l/ml) and TGF- (5 l/ml) or control antibodies before addition to PBMC cultures. Dedication of Cytokine Concentrations in CM and Sera IFN-, IL-10, and IL-4 protein concentrations were dependant on enzyme-linked immunosorbent assay (ELISA),… Continue reading In decided on experiments, cell line supernatants were preincubated for one hour at space temperature with neutralizing antisera to IL-10 (10 l/ml) and TGF- (5 l/ml) or control antibodies before addition to PBMC cultures

Error bars show standard deviation. response to drugs, correlating with reduced p53 apoptotic transactivation. The drug-induced apoptotic cell death, reduced by high glucose, was finally restored by the phosphatase inhibitor calyculin A. Conclusions These data indicate that high glucose specifically inhibited Ser46 phosphorylation thus reducing p53 apoptotic activity. These results uncover a new mechanism of… Continue reading Error bars show standard deviation

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