Cell-traversal protein for ookinetes and sporozoites (CelTOS) and circumsporozoite protein (CSP) are important sporozoite antigens that are relatively more conserved compared to merozoite surface antigens [14-16] and may be ideal candidates for estimating malaria transmission intensity. transmission area of Southern Ghana were assessed by indirect ELISA. Seroprevalence of antibodies against CSP, CelTOS and AMA1 were fitted to reversible catalytic models to estimate and corresponding seroreversion rates () for each antibody. Results Of the three models developed, the anti-CSP model predicted a 13-fold decrease in four years prior to the time of sampling (2009). Anti-AMA1 antibodies formed at a four-fold greater rate compared to that of anti-CelTOS Rolapitant antibodies, and anti-CSP antibodies during the period of decreased . In contrast, anti-AMA1 antibodies decayed at a five-fold slower rate relative KITH_HHV1 antibody to that of anti-CSP antibodies while anti-AMA1 and anti-CelTOS antibody decay rates were not significantly different. Anti-CSP antibodies were relatively short-lived as they formed at an 11.6-fold slower rate relative to their decay during the period of decreased . Conclusions These features of anti-CSP antibodies can be exploited for the development of models for predicting seasonal, short-term changes in transmission intensity in malaria-endemic areas, especially as the elimination phase of malaria Rolapitant control is usually approached. Keywords: Malaria, Antibody seroconversion rate, Seroreversion rate, Transmission intensity, Sporozoite antigens, ELISA Background Malaria caused by is an infectious disease of public health importance, with an estimated mortality of 655,000 in 2010 2010 [1]. The most severe forms of malaria are usually caused by elicits immune responses that confer an age and exposure-dependent semi-immunity to infected individuals while being indicative of exposure to parasites. The prevalence of antibodies against such antigens as apical membrane antigen 1 (AMA1), the 19?kDa fragment of merozoite surface protein 1 (MSP119) and merozoite surface protein 2 (MSP2) have gained relevance as transmission monitoring tools [8-10]. In areas of stable medium to high transmission, antibody prevalence estimates correlate well with standard EIR estimates, but have an advantage over EIR estimation since antibody decay is usually slower than parasite clearance rates. The persistence of antibodies long after transmission has ceased however represents Rolapitant a weakness in this approach [11-13], especially if models are to be used for the prediction of seasonal or short-term changes in transmission. A careful selection of antigens and adjustment for antibody persistence in estimation models are therefore necessary under these conditions. Unlike merozoites, the sporozoite stages of are exposed to the immune system for only Rolapitant short periods after inoculation, and anti-sporozoite antibodies would most commonly be detected in individuals with frequent or recent exposure. Cell-traversal protein for ookinetes Rolapitant and sporozoites (CelTOS) and circumsporozoite protein (CSP) are important sporozoite antigens that are relatively more conserved compared to merozoite surface antigens [14-16] and could be ideal applicants for estimating malaria transmitting intensity. Estimates predicated on these antigens, that have brief immune publicity times, might consequently better assess variations in contact with parasites while removing the necessity for taking into consideration long-term antibody persistence and antigen polymorphism. This process shall also measure immediate contact with sporozoites like the available EIR yellow metal regular, instead of versions based on bloodstream stage antigens, which might not reflect immediate sporozoite publicity. A precise estimation of malaria transmitting intensity is vital since it will permit an evaluation of the potency of interventions and assist in preparing in the platform from the limited assets that exist to many disease-endemic countries in order that current benefits aren’t eroded. The purpose of this scholarly study was.