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[PubMed] [Google Scholar] 24. death when 3-BrPA and CQ were co-administered. Finally, CQ enhanced the anticancer effectiveness of 3-BrPA and tumor experiment The nude mice (5-6 weeks) used in these studies were from Beijing vitalriver and weighed 20C25 g at the time of tumor implantation. The mice were kept under a 12:12 h lightCdark cycle, at 24 2 C and fed with clean food and water. Human being MDA-MB-231 cells (107cells/ml) were inoculated subcutaneously to form tumors. Mice with tumors (100-200 mm3) assorted to four organizations (5 mice/group). Vehicle (0.9% NS) or CQ (40mg/kg/d, 24 days) or 3-BrPA (5mg/kg/d, 24 days) alone or in combination was administrated Methoxatin disodium salt intraperitoneally. Tumor growth was monitored every three days by two-dimensional measurements of individual tumors for each mouse. Tumor volume was determined using the method: lengthwidth2/2. After treatment ended, mice from each group were sacrificed. Tumors were excised, determined and fixed in 4% formalin answer, inlayed in paraffin, and then stained with hematoxillin-eosin (H&E). Statistical analysis Statistical analyses were performed with Concise Statistics Methoxatin disodium salt (CS 14.0) software. The data offered were mean standard deviation (SD). Data were compared using Student’s t test. p< 0.05 was considered significant. Acknowledgments This work was supported from the National Natural Science Basis of China (81000992, 81072207) and the Natural Science Basis of Anhui Province (090413135), Education Division of Anhui Natural Science Research Important Project China (KJ2012A202). Contributed by QWZ carried out the experiments and published the manuscript. YYZ, PZ, FX, ZHC participated in the design and coordination of study. CCJ, ZWJ, HL conceived of the study, participated in the design and helped to draft the manuscript. All authors read and authorized the final manuscript. The authors declare that they have no competing interests. Recommendations 1. Warburg O. On respiratory impairment in malignancy cells. Technology. 1956;124:267C9. [PubMed] [Google Scholar] 2. Moreno-S?anchez R, Rodr??guez-Enr??quez S, Mar?? n-Hern?andez A, Saavedra E. Energy rate of metabolism in tumor cells. FEBS. 2007;274:1393C418. [PubMed] [Google Scholar] 3. Pedersen P. The malignancy cell's power vegetation as promising restorative targets an overview. Bioenerg Biomembr. 2007;39:1C12. [PubMed] [Google Scholar] 4. Dwarakanath BS. Cytotoxicity, radiosensitization, and chemosensitization of tumor cells by 2-deoxy-D-glucose in vitro. Malignancy Res Ther. 2009;5:S27CS31. [PubMed] [Google Scholar] 5. Mathupala SP, Ko Y. H, Pedersen PL. Hexokinase-2 bound to mitochondria: cancer's stygian link to the Warburg Effect and a pivotal target for effective therapy. Semin Malignancy Biol. 2009;19:17C24. [PMC free article] [PubMed] [Google Scholar] 6. Nelson K. 3-bromopyruvate kills malignancy cells in animals. Lancet Oncol. 2002;3:524. [PubMed] [Google Scholar] 7. Geschwind JF, Ko YH, Torbenson MS, Magee C, Pedersen PL. Novel therapy for liver cancer: direct intra arterial injection of a potent inhibitor of ATP production. Malignancy Res. 2002;62:3909C13. [PubMed] [Google Scholar] 8. Patra KC, Hay N. Hexokinase 2 as oncotarget. Oncotarget. 2013;4:1862C3. [PMC free article] [PubMed] [Google Scholar] 9. Patra KC, Wang Q, Bhaskar PT, Miller L, Wang Z, Wheaton W, Chandel N, Laakso M, Muller WJ, Allen EL, Jha AK, Smolen GA, Clasquin MF, Robey RB, Hay N. Hexokinase 2 is required for tumor initiation and maintenance and its systemic deletion is FLJ30619 definitely restorative in Methoxatin disodium salt mouse models of malignancy. Malignancy Cell. 2013;24:213C28. [PMC free article] [PubMed] [Google Scholar] 10. Marini C1, Salani B, Massollo M, Amaro A, Esposito AI, Orengo AM, Capitanio S, Emionite L, Riondato M, Bottoni G, Massara C, Boccardo S, Fabbi M, Campi C, Ravera S, Angelini G, Morbelli S, Cilli M, Cordera R, Truini M, Maggi D, Pfeffer U, Sambuceti G. Direct inhibition of hexokinase activity by metformin at least partially impairs glucose rate of metabolism and tumor growth in experimental breast cancer. Cell Cycle. 2013;12:3490C9. [PMC free article] [PubMed] [Google Scholar] 11. Ko YH, Pedersen PL, Geschwind JF. Glucose catabolism in the rabbit VX2 tumor model for liver malignancy: characterization and focusing on hexokinase. Malignancy Lett. 2001;173:83C91. [PubMed] [Google Scholar] 12. Xu R, Pelicano YZ, Carew JS, Feng L, Bhalla KN, Keating MJ, Huang P. Inhibition of glycolysis in malignancy cells: a novel strategy to conquer compound resistance associated with mitochondrial respiratory defect and hypoxia. Malignancy Re..